Lawes picked up a pectoral injury in training ahead of Saturday's clash with Wales in Cardiff.
And England attack coach Simon Amor has now revealed that the problem will see Lawes take no further part in the tournament.
Speaking on Friday afternoon, Amor said: "Courtney picked up a knock on Wednesday and that means he won’t be available for us for the rest of the Six Nations.
"It was a fall, he tweaked his pec.
"It’s obviously very disappointing but these things happen and we’ll look at the replacement after the Wales game.”